The Recombinant Protein Handbook Protein Amplification and Simple Purification 18-1142-75 Protein Purification

نویسنده

  • A. N. de Belder
چکیده

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

18-1121-86 AF HE.pmd

Information ColumnsPack sizeCode No. HiTrap rProtein A FF5 × 1 ml17-5079-01 HiTrap rProtein A FF2 × 1 ml17-5079-02 HiTrap rProtein A FF1 × 5 ml17-5080-01 HiTrap Protein A HP5 × 1 ml17-0402-01 HiTrap Protein A HP2 × 1 ml17-0402-03 HiTrap Protein A HP1 × 5 ml17-0403-01 HiTrap Protein G HP5 × 1 ml17-0404-01 HiTrap Protein G HP2 × 1 ml17-0404-03 HiTrap Protein G HP1 × 5 ml17-0405-01 HiTrap Blue HP5...

متن کامل

Design and Construction of Recombinant ELP-Intein Cassette for Use in Simple and new Purification Methods of Recombinant Proteins

Background and Objective: Use of elastin-like proteins (ELPs) provides high-performance protein purification without need for chromatography. In line with cost reduction and facilitation of recombinant proteins purification, which represent a high percentage of production costs, in this project, we eliminated the need for proteases in the process of separation of recombinant proteins from ELP b...

متن کامل

Recombinant production and affinity purification of the FraC pore forming toxin using hexa-His tag and pET expression cassette

Objective(s): A newly-introduced protein toxin from a sea anemone, namely fragaceatoxin C is a protein with molecular weight of 20 kDa and pore-forming capability against cell membranes has recently grasped great attentions for its function. In this study, its coding sequence cloned as a fusion protein with His-tag for simple production and rapid purification. Materials and Methods: After PCR a...

متن کامل

Amplification, cloning and expression of Brucella melitensis bp26 gene (OMP28) isolated from Markazi province (Iran) and purification of Bp26 Protein

Brucellosis is a debilitative disease that imposes costs on both economy and society. It is shown that although the vaccine can prevent abortion, it does not provide complete protection against infection. In Iran, Brucella melitensis is a common causative agent for brucellosis and BP26 protein of this bacterium having a good antigenesity and an important vaccine candidate. In this study B. meli...

متن کامل

Expression and Simple Purification Strategy for the Generation of Anti-microbial Active Enterocin P from Enterococcus faecium Expressed in Escherichia coli ER2566

Background: Enterocin-P of Enterococcus faecium P13 (EntP) is of great interest as a food preservative and medicine due to its non-toxicity and broad antimicrobial spectrum in various pH, as well as its excellent thermal stability. However, recombinant production of EntP is still in laboratory because of low productivity and complex purification process. Objectives: In this study, we aimed to ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2003